fvb mice Search Results


mdr1a 1b ko mice  (Taconic Biosciences)
93
Taconic Biosciences mdr1a 1b ko mice
Mdr1a 1b Ko Mice, supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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mrp1 deficient  (Taconic Biosciences)
86
Taconic Biosciences mrp1 deficient
Mrp1 Deficient, supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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female fvb inbred mice  (Envigo)
93
Envigo female fvb inbred mice
Female Fvb Inbred Mice, supplied by Envigo, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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female fvb wild type  (Taconic Biosciences)
95
Taconic Biosciences female fvb wild type
Female Fvb Wild Type, supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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friend leukemia virus strain b fvb wild type  (Taconic Biosciences)
95
Taconic Biosciences friend leukemia virus strain b fvb wild type
Total plasma (solid line with square) concentration and brain (dashed line with circle) concentration in <t>wild-type</t> mice (A) and Mdr1a/b(−/−)Bcrp1(−/−) mice (B), and brain-to-plasma ratio time course of ponatinib after administration of a single intravenous bolus (3 mg/kg) in <t>FVB</t> wild-type and Mdr1a/b(−/−)Bcrp1(−/−) mice (N = 3 to 4 at each time point) (C). Data are presented as the mean ± S.D.
Friend Leukemia Virus Strain B Fvb Wild Type, supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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fvb inbred mice  (Charles River Laboratories)
90
Charles River Laboratories fvb inbred mice
Total plasma (solid line with square) concentration and brain (dashed line with circle) concentration in <t>wild-type</t> mice (A) and Mdr1a/b(−/−)Bcrp1(−/−) mice (B), and brain-to-plasma ratio time course of ponatinib after administration of a single intravenous bolus (3 mg/kg) in <t>FVB</t> wild-type and Mdr1a/b(−/−)Bcrp1(−/−) mice (N = 3 to 4 at each time point) (C). Data are presented as the mean ± S.D.
Fvb Inbred Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fvb female mice  (Harlan Laboratories)
90
Harlan Laboratories fvb female mice
Total plasma (solid line with square) concentration and brain (dashed line with circle) concentration in <t>wild-type</t> mice (A) and Mdr1a/b(−/−)Bcrp1(−/−) mice (B), and brain-to-plasma ratio time course of ponatinib after administration of a single intravenous bolus (3 mg/kg) in <t>FVB</t> wild-type and Mdr1a/b(−/−)Bcrp1(−/−) mice (N = 3 to 4 at each time point) (C). Data are presented as the mean ± S.D.
Fvb Female Mice, supplied by Harlan Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transgenic mice (fvb-tg(mmtv-erbb2)nk1mul/j  (Jackson Laboratory)
90
Jackson Laboratory transgenic mice (fvb-tg(mmtv-erbb2)nk1mul/j
A. Breeding strategy used to create experimental cohorts. Two generation breeding was used to produce tumor-prone MMTV-NeuNT (activated rat <t>ERBB2)</t> <t>transgenic</t> MARKO (male and females underlined on the left side) and Control (underlined on the right) mice. MARKO mice are positive for MMTV-cre transgene, specifically expressed in mammary glands. B. Recombination events in the genomic DNA from mammary ductal tissue dissociated from fat cells. Top band (952 bp) is derived from the non-recombined floxed Ar fl allele, middle band (855 bp) is from the wild-type Ar + allele, and the lower band (404 bp) is an amplicon derived from the Ar Δ allele, resulting from Cre/LoxP induced deletion of exon 2. Ar fl /+ is a positive control for the floxed allele and WT is wild-type ( Ar + /Ar + ). Left panel is recombination in male mice and the right panel is recombination in female mice. No recombination is observed in mice lacking MMTV-cre (lanes 1 and 4), the deleted allele is present in mice with MMTV-cre (lanes 2 and 3) C. Reduced number of AR positive luminal epithelial cells in MARKO mammary glands. AR positive luminal epithelial cells in the mammary glands of Control (n = 5) and MARKO (n = 5) mice were counted and determined as a percentage of the total number of cells per gland (p = 0.0019). An average of 260 cells were counted per individual mouse. Immunohistochemical staining for AR expression in Control (D) and MARKO (E and F) mammary glands. Scale bar = 20 µm.
Transgenic Mice (Fvb Tg(Mmtv Erbb2)Nk1mul/J, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transgenic mice (fvb-tg(mmtv-erbb2)nk1mul/j/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
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fvb mice  (Jackson Laboratory)
90
Jackson Laboratory fvb mice
A. Breeding strategy used to create experimental cohorts. Two generation breeding was used to produce tumor-prone MMTV-NeuNT (activated rat <t>ERBB2)</t> <t>transgenic</t> MARKO (male and females underlined on the left side) and Control (underlined on the right) mice. MARKO mice are positive for MMTV-cre transgene, specifically expressed in mammary glands. B. Recombination events in the genomic DNA from mammary ductal tissue dissociated from fat cells. Top band (952 bp) is derived from the non-recombined floxed Ar fl allele, middle band (855 bp) is from the wild-type Ar + allele, and the lower band (404 bp) is an amplicon derived from the Ar Δ allele, resulting from Cre/LoxP induced deletion of exon 2. Ar fl /+ is a positive control for the floxed allele and WT is wild-type ( Ar + /Ar + ). Left panel is recombination in male mice and the right panel is recombination in female mice. No recombination is observed in mice lacking MMTV-cre (lanes 1 and 4), the deleted allele is present in mice with MMTV-cre (lanes 2 and 3) C. Reduced number of AR positive luminal epithelial cells in MARKO mammary glands. AR positive luminal epithelial cells in the mammary glands of Control (n = 5) and MARKO (n = 5) mice were counted and determined as a percentage of the total number of cells per gland (p = 0.0019). An average of 260 cells were counted per individual mouse. Immunohistochemical staining for AR expression in Control (D) and MARKO (E and F) mammary glands. Scale bar = 20 µm.
Fvb Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fvb mice/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
fvb mice - by Bioz Stars, 2026-03
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fvb  (Janvier Labs)
90
Janvier Labs fvb
A. Breeding strategy used to create experimental cohorts. Two generation breeding was used to produce tumor-prone MMTV-NeuNT (activated rat <t>ERBB2)</t> <t>transgenic</t> MARKO (male and females underlined on the left side) and Control (underlined on the right) mice. MARKO mice are positive for MMTV-cre transgene, specifically expressed in mammary glands. B. Recombination events in the genomic DNA from mammary ductal tissue dissociated from fat cells. Top band (952 bp) is derived from the non-recombined floxed Ar fl allele, middle band (855 bp) is from the wild-type Ar + allele, and the lower band (404 bp) is an amplicon derived from the Ar Δ allele, resulting from Cre/LoxP induced deletion of exon 2. Ar fl /+ is a positive control for the floxed allele and WT is wild-type ( Ar + /Ar + ). Left panel is recombination in male mice and the right panel is recombination in female mice. No recombination is observed in mice lacking MMTV-cre (lanes 1 and 4), the deleted allele is present in mice with MMTV-cre (lanes 2 and 3) C. Reduced number of AR positive luminal epithelial cells in MARKO mammary glands. AR positive luminal epithelial cells in the mammary glands of Control (n = 5) and MARKO (n = 5) mice were counted and determined as a percentage of the total number of cells per gland (p = 0.0019). An average of 260 cells were counted per individual mouse. Immunohistochemical staining for AR expression in Control (D) and MARKO (E and F) mammary glands. Scale bar = 20 µm.
Fvb, supplied by Janvier Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fvb/product/Janvier Labs
Average 90 stars, based on 1 article reviews
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90/100 stars
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mmtv/r-neu fvb mice (h-2q) transgenic for the rat neu protein (rneutg)  (Charles River Laboratories)
90
Charles River Laboratories mmtv/r-neu fvb mice (h-2q) transgenic for the rat neu protein (rneutg)
A. Breeding strategy used to create experimental cohorts. Two generation breeding was used to produce tumor-prone MMTV-NeuNT (activated rat <t>ERBB2)</t> <t>transgenic</t> MARKO (male and females underlined on the left side) and Control (underlined on the right) mice. MARKO mice are positive for MMTV-cre transgene, specifically expressed in mammary glands. B. Recombination events in the genomic DNA from mammary ductal tissue dissociated from fat cells. Top band (952 bp) is derived from the non-recombined floxed Ar fl allele, middle band (855 bp) is from the wild-type Ar + allele, and the lower band (404 bp) is an amplicon derived from the Ar Δ allele, resulting from Cre/LoxP induced deletion of exon 2. Ar fl /+ is a positive control for the floxed allele and WT is wild-type ( Ar + /Ar + ). Left panel is recombination in male mice and the right panel is recombination in female mice. No recombination is observed in mice lacking MMTV-cre (lanes 1 and 4), the deleted allele is present in mice with MMTV-cre (lanes 2 and 3) C. Reduced number of AR positive luminal epithelial cells in MARKO mammary glands. AR positive luminal epithelial cells in the mammary glands of Control (n = 5) and MARKO (n = 5) mice were counted and determined as a percentage of the total number of cells per gland (p = 0.0019). An average of 260 cells were counted per individual mouse. Immunohistochemical staining for AR expression in Control (D) and MARKO (E and F) mammary glands. Scale bar = 20 µm.
Mmtv/R Neu Fvb Mice (H 2q) Transgenic For The Rat Neu Protein (Rneutg), supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mmtv/r-neu fvb mice (h-2q) transgenic for the rat neu protein (rneutg)/product/Charles River Laboratories
Average 90 stars, based on 1 article reviews
mmtv/r-neu fvb mice (h-2q) transgenic for the rat neu protein (rneutg) - by Bioz Stars, 2026-03
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male fvb mice with indwelling intracerebroventricular (icv) catheters  (Charles River Laboratories)
90
Charles River Laboratories male fvb mice with indwelling intracerebroventricular (icv) catheters
A. Breeding strategy used to create experimental cohorts. Two generation breeding was used to produce tumor-prone MMTV-NeuNT (activated rat <t>ERBB2)</t> <t>transgenic</t> MARKO (male and females underlined on the left side) and Control (underlined on the right) mice. MARKO mice are positive for MMTV-cre transgene, specifically expressed in mammary glands. B. Recombination events in the genomic DNA from mammary ductal tissue dissociated from fat cells. Top band (952 bp) is derived from the non-recombined floxed Ar fl allele, middle band (855 bp) is from the wild-type Ar + allele, and the lower band (404 bp) is an amplicon derived from the Ar Δ allele, resulting from Cre/LoxP induced deletion of exon 2. Ar fl /+ is a positive control for the floxed allele and WT is wild-type ( Ar + /Ar + ). Left panel is recombination in male mice and the right panel is recombination in female mice. No recombination is observed in mice lacking MMTV-cre (lanes 1 and 4), the deleted allele is present in mice with MMTV-cre (lanes 2 and 3) C. Reduced number of AR positive luminal epithelial cells in MARKO mammary glands. AR positive luminal epithelial cells in the mammary glands of Control (n = 5) and MARKO (n = 5) mice were counted and determined as a percentage of the total number of cells per gland (p = 0.0019). An average of 260 cells were counted per individual mouse. Immunohistochemical staining for AR expression in Control (D) and MARKO (E and F) mammary glands. Scale bar = 20 µm.
Male Fvb Mice With Indwelling Intracerebroventricular (Icv) Catheters, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/male fvb mice with indwelling intracerebroventricular (icv) catheters/product/Charles River Laboratories
Average 90 stars, based on 1 article reviews
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Image Search Results


Total plasma (solid line with square) concentration and brain (dashed line with circle) concentration in wild-type mice (A) and Mdr1a/b(−/−)Bcrp1(−/−) mice (B), and brain-to-plasma ratio time course of ponatinib after administration of a single intravenous bolus (3 mg/kg) in FVB wild-type and Mdr1a/b(−/−)Bcrp1(−/−) mice (N = 3 to 4 at each time point) (C). Data are presented as the mean ± S.D.

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Pharmacokinetic Assessment of Cooperative Efflux of the Multitargeted Kinase Inhibitor Ponatinib Across the Blood-Brain Barrier

doi: 10.1124/jpet.117.246116

Figure Lengend Snippet: Total plasma (solid line with square) concentration and brain (dashed line with circle) concentration in wild-type mice (A) and Mdr1a/b(−/−)Bcrp1(−/−) mice (B), and brain-to-plasma ratio time course of ponatinib after administration of a single intravenous bolus (3 mg/kg) in FVB wild-type and Mdr1a/b(−/−)Bcrp1(−/−) mice (N = 3 to 4 at each time point) (C). Data are presented as the mean ± S.D.

Article Snippet: Animals Pharmacokinetic studies were conducted using Friend leukemia virus strain B (FVB) wild-type, Bcrp1(−/−) , Mdr1a/b(−/−) , and Mdr1a/b(−/−)Bcrp1(−/−) mice (Taconic Biosciences, Inc., Germantown, NY).

Techniques: Concentration Assay

Pharmacokinetic/metric parameters estimated from NCA of total brain and plasma concentration-time profiles after administration of a single intravenous bolus of ponatinib (3 mg/kg) in FVB wild-type and Mdr1a/b(−/−)Bcrp1(−/−) mice ( N = 3 to 4 at each time point) Data are presented as mean or mean ± S.E.M, unless otherwise indicated.

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Pharmacokinetic Assessment of Cooperative Efflux of the Multitargeted Kinase Inhibitor Ponatinib Across the Blood-Brain Barrier

doi: 10.1124/jpet.117.246116

Figure Lengend Snippet: Pharmacokinetic/metric parameters estimated from NCA of total brain and plasma concentration-time profiles after administration of a single intravenous bolus of ponatinib (3 mg/kg) in FVB wild-type and Mdr1a/b(−/−)Bcrp1(−/−) mice ( N = 3 to 4 at each time point) Data are presented as mean or mean ± S.E.M, unless otherwise indicated.

Article Snippet: Animals Pharmacokinetic studies were conducted using Friend leukemia virus strain B (FVB) wild-type, Bcrp1(−/−) , Mdr1a/b(−/−) , and Mdr1a/b(−/−)Bcrp1(−/−) mice (Taconic Biosciences, Inc., Germantown, NY).

Techniques: Concentration Assay, Mouse Assay

Total plasma (solid line with square) and brain (dashed line with circle) concentration-time profiles after administration of a single oral dose (30 mg/kg) of ponatinib in FVB wild-type (A), Bcrp1(−/−) (B), Mdr1a/b(−/−) (C), and Mdr1a/b(−/−)Bcrp1(−/) (D) mice (N = 4 at each time point). Data are presented as mean ± S.D. Data for the wild-type mice were previously reported (Laramy et al., 2017) and included in this present study to compare the wild-type genotype with the three other genotypes that lack efflux transporters.

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Pharmacokinetic Assessment of Cooperative Efflux of the Multitargeted Kinase Inhibitor Ponatinib Across the Blood-Brain Barrier

doi: 10.1124/jpet.117.246116

Figure Lengend Snippet: Total plasma (solid line with square) and brain (dashed line with circle) concentration-time profiles after administration of a single oral dose (30 mg/kg) of ponatinib in FVB wild-type (A), Bcrp1(−/−) (B), Mdr1a/b(−/−) (C), and Mdr1a/b(−/−)Bcrp1(−/) (D) mice (N = 4 at each time point). Data are presented as mean ± S.D. Data for the wild-type mice were previously reported (Laramy et al., 2017) and included in this present study to compare the wild-type genotype with the three other genotypes that lack efflux transporters.

Article Snippet: Animals Pharmacokinetic studies were conducted using Friend leukemia virus strain B (FVB) wild-type, Bcrp1(−/−) , Mdr1a/b(−/−) , and Mdr1a/b(−/−)Bcrp1(−/−) mice (Taconic Biosciences, Inc., Germantown, NY).

Techniques: Concentration Assay

Total brain-to-plasma ratio profiles after administration of a single oral dose (30 mg/kg) in FVB wild-type, Bcrp1(−/−), Mdr1a/b(−/−), and Mdr1a/b(−/−)Bcrp1(−/−) mice (N = 4 at each time point). Data are presented as the mean ± S.D. Data for the wild-type were previously reported (Laramy et al., 2017) and were included in this present study to compare the wild-type genotype with the three other genotypes that lack efflux transporters.

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Pharmacokinetic Assessment of Cooperative Efflux of the Multitargeted Kinase Inhibitor Ponatinib Across the Blood-Brain Barrier

doi: 10.1124/jpet.117.246116

Figure Lengend Snippet: Total brain-to-plasma ratio profiles after administration of a single oral dose (30 mg/kg) in FVB wild-type, Bcrp1(−/−), Mdr1a/b(−/−), and Mdr1a/b(−/−)Bcrp1(−/−) mice (N = 4 at each time point). Data are presented as the mean ± S.D. Data for the wild-type were previously reported (Laramy et al., 2017) and were included in this present study to compare the wild-type genotype with the three other genotypes that lack efflux transporters.

Article Snippet: Animals Pharmacokinetic studies were conducted using Friend leukemia virus strain B (FVB) wild-type, Bcrp1(−/−) , Mdr1a/b(−/−) , and Mdr1a/b(−/−)Bcrp1(−/−) mice (Taconic Biosciences, Inc., Germantown, NY).

Techniques:

Pharmacokinetic/metric parameters determined by NCA of total brain and plasma concentration-time profiles after a single oral dose (30 mg/kg) of ponatinib in FVB wild-type, Bcrp1(−/−) , Mdr1a/b(−/−) , and Mdr1a/b(−/−)Bcrp1(−/−) mice ( N = 4 at each time point) Data are presented as the mean or mean ± S.E.M, unless otherwise indicated. Data for the wild-type mice were previously reported ( Laramy et al., 2017 ) and are included in this present study to compare with the three other genotypes that lack efflux transporters.

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Pharmacokinetic Assessment of Cooperative Efflux of the Multitargeted Kinase Inhibitor Ponatinib Across the Blood-Brain Barrier

doi: 10.1124/jpet.117.246116

Figure Lengend Snippet: Pharmacokinetic/metric parameters determined by NCA of total brain and plasma concentration-time profiles after a single oral dose (30 mg/kg) of ponatinib in FVB wild-type, Bcrp1(−/−) , Mdr1a/b(−/−) , and Mdr1a/b(−/−)Bcrp1(−/−) mice ( N = 4 at each time point) Data are presented as the mean or mean ± S.E.M, unless otherwise indicated. Data for the wild-type mice were previously reported ( Laramy et al., 2017 ) and are included in this present study to compare with the three other genotypes that lack efflux transporters.

Article Snippet: Animals Pharmacokinetic studies were conducted using Friend leukemia virus strain B (FVB) wild-type, Bcrp1(−/−) , Mdr1a/b(−/−) , and Mdr1a/b(−/−)Bcrp1(−/−) mice (Taconic Biosciences, Inc., Germantown, NY).

Techniques: Concentration Assay, Mouse Assay

Total steady-state plasma and brain concentrations (A) and corresponding total steady-state brain-to-plasma ratios (B) of ponatinib after continuous intraperitoneal infusion (40 µg/h) for 48 hours in FVB wild-type, Bcrp1(−/−), Mdr1a/b(−/−), and Mdr1a/b(−/−)Bcrp1(−/−) mice (N = 4 in each genotype) (*P < 0.05; *statistical significance). Data are presented as the mean ± S.D.

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Pharmacokinetic Assessment of Cooperative Efflux of the Multitargeted Kinase Inhibitor Ponatinib Across the Blood-Brain Barrier

doi: 10.1124/jpet.117.246116

Figure Lengend Snippet: Total steady-state plasma and brain concentrations (A) and corresponding total steady-state brain-to-plasma ratios (B) of ponatinib after continuous intraperitoneal infusion (40 µg/h) for 48 hours in FVB wild-type, Bcrp1(−/−), Mdr1a/b(−/−), and Mdr1a/b(−/−)Bcrp1(−/−) mice (N = 4 in each genotype) (*P < 0.05; *statistical significance). Data are presented as the mean ± S.D.

Article Snippet: Animals Pharmacokinetic studies were conducted using Friend leukemia virus strain B (FVB) wild-type, Bcrp1(−/−) , Mdr1a/b(−/−) , and Mdr1a/b(−/−)Bcrp1(−/−) mice (Taconic Biosciences, Inc., Germantown, NY).

Techniques:

Observed (red square) and model-predicted (red solid line) total plasma concentration-time profiles, and observed (black circle) and model-predicted (black dashed line) total brain concentration-time profiles in FVB wild-type (A) and Mdr1a/b(−/−)Bcrp1(−/−) (B) mice (N = 3 to 4 at each time point) after a single intravenous bolus (3 mg/kg). The observed data are presented as the mean ± S.D.

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Pharmacokinetic Assessment of Cooperative Efflux of the Multitargeted Kinase Inhibitor Ponatinib Across the Blood-Brain Barrier

doi: 10.1124/jpet.117.246116

Figure Lengend Snippet: Observed (red square) and model-predicted (red solid line) total plasma concentration-time profiles, and observed (black circle) and model-predicted (black dashed line) total brain concentration-time profiles in FVB wild-type (A) and Mdr1a/b(−/−)Bcrp1(−/−) (B) mice (N = 3 to 4 at each time point) after a single intravenous bolus (3 mg/kg). The observed data are presented as the mean ± S.D.

Article Snippet: Animals Pharmacokinetic studies were conducted using Friend leukemia virus strain B (FVB) wild-type, Bcrp1(−/−) , Mdr1a/b(−/−) , and Mdr1a/b(−/−)Bcrp1(−/−) mice (Taconic Biosciences, Inc., Germantown, NY).

Techniques: Concentration Assay

Observed (red square) and model-predicted (red solid line) total plasma concentration-time profiles, and observed (black circle) and model-predicted (black dashed line) total brain concentration-time profiles in FVB wild-type (A), Bcrp1(−/−) (B), Mdr1a/b(−/−) (C), and Mdr1a/b(−/−)Bcrp1(−/−) (D) mice (N = 4 at each time point) after a single oral dose (30 mg/kg). The observed data are presented as the mean ± S.D. The observed data for the wild-type were previously reported (Laramy et al., 2017) and were included in this present study to compare with the three other genotypes that lack efflux transporters.

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Pharmacokinetic Assessment of Cooperative Efflux of the Multitargeted Kinase Inhibitor Ponatinib Across the Blood-Brain Barrier

doi: 10.1124/jpet.117.246116

Figure Lengend Snippet: Observed (red square) and model-predicted (red solid line) total plasma concentration-time profiles, and observed (black circle) and model-predicted (black dashed line) total brain concentration-time profiles in FVB wild-type (A), Bcrp1(−/−) (B), Mdr1a/b(−/−) (C), and Mdr1a/b(−/−)Bcrp1(−/−) (D) mice (N = 4 at each time point) after a single oral dose (30 mg/kg). The observed data are presented as the mean ± S.D. The observed data for the wild-type were previously reported (Laramy et al., 2017) and were included in this present study to compare with the three other genotypes that lack efflux transporters.

Article Snippet: Animals Pharmacokinetic studies were conducted using Friend leukemia virus strain B (FVB) wild-type, Bcrp1(−/−) , Mdr1a/b(−/−) , and Mdr1a/b(−/−)Bcrp1(−/−) mice (Taconic Biosciences, Inc., Germantown, NY).

Techniques: Concentration Assay

A. Breeding strategy used to create experimental cohorts. Two generation breeding was used to produce tumor-prone MMTV-NeuNT (activated rat ERBB2) transgenic MARKO (male and females underlined on the left side) and Control (underlined on the right) mice. MARKO mice are positive for MMTV-cre transgene, specifically expressed in mammary glands. B. Recombination events in the genomic DNA from mammary ductal tissue dissociated from fat cells. Top band (952 bp) is derived from the non-recombined floxed Ar fl allele, middle band (855 bp) is from the wild-type Ar + allele, and the lower band (404 bp) is an amplicon derived from the Ar Δ allele, resulting from Cre/LoxP induced deletion of exon 2. Ar fl /+ is a positive control for the floxed allele and WT is wild-type ( Ar + /Ar + ). Left panel is recombination in male mice and the right panel is recombination in female mice. No recombination is observed in mice lacking MMTV-cre (lanes 1 and 4), the deleted allele is present in mice with MMTV-cre (lanes 2 and 3) C. Reduced number of AR positive luminal epithelial cells in MARKO mammary glands. AR positive luminal epithelial cells in the mammary glands of Control (n = 5) and MARKO (n = 5) mice were counted and determined as a percentage of the total number of cells per gland (p = 0.0019). An average of 260 cells were counted per individual mouse. Immunohistochemical staining for AR expression in Control (D) and MARKO (E and F) mammary glands. Scale bar = 20 µm.

Journal: PLoS ONE

Article Title: Reduced Androgen Receptor Expression Accelerates the Onset of ERBB2 Induced Breast Tumors in Female Mice

doi: 10.1371/journal.pone.0060455

Figure Lengend Snippet: A. Breeding strategy used to create experimental cohorts. Two generation breeding was used to produce tumor-prone MMTV-NeuNT (activated rat ERBB2) transgenic MARKO (male and females underlined on the left side) and Control (underlined on the right) mice. MARKO mice are positive for MMTV-cre transgene, specifically expressed in mammary glands. B. Recombination events in the genomic DNA from mammary ductal tissue dissociated from fat cells. Top band (952 bp) is derived from the non-recombined floxed Ar fl allele, middle band (855 bp) is from the wild-type Ar + allele, and the lower band (404 bp) is an amplicon derived from the Ar Δ allele, resulting from Cre/LoxP induced deletion of exon 2. Ar fl /+ is a positive control for the floxed allele and WT is wild-type ( Ar + /Ar + ). Left panel is recombination in male mice and the right panel is recombination in female mice. No recombination is observed in mice lacking MMTV-cre (lanes 1 and 4), the deleted allele is present in mice with MMTV-cre (lanes 2 and 3) C. Reduced number of AR positive luminal epithelial cells in MARKO mammary glands. AR positive luminal epithelial cells in the mammary glands of Control (n = 5) and MARKO (n = 5) mice were counted and determined as a percentage of the total number of cells per gland (p = 0.0019). An average of 260 cells were counted per individual mouse. Immunohistochemical staining for AR expression in Control (D) and MARKO (E and F) mammary glands. Scale bar = 20 µm.

Article Snippet: Transgenic mice (FVB-Tg(MMTV-ErbB2)NK1Mul/J) containing the activated rat ERBB2 oncogene targeted to the mammary epithelium by the MMTV-LTR promoter (hereafter referred to as MMTV-NeuNT) and mice with the Cre recombinase transgene under the control of the MMTV-LTR promoter (Tg(MMTV-cre)1Mam/J, MMTV-cre) were purchased from Jackson Laboratories (Bar Harbor, ME, USA).

Techniques: Transgenic Assay, Control, Derivative Assay, Amplification, Positive Control, Immunohistochemical staining, Staining, Expressing